Use liquid biopsies to expand the applications of your PDX mouse models
The RareCyte platform enables you to expand the scope of your research with Patient Derived Xenograft (PDX) mice and to undertake new kinds of studies. By replacing tumor biopsies with liquid biopsies, you spend less time gathering samples and can analyze many more. In addition, you can perform repeated assays on the same mouse, which enhances the validity of your sample-to-sample comparisons.
Working with liquid biopsy samples from mice has been problematic because so little sample material is typically obtained from any given mouse. The high sensitivity of RareCyte platform virtually eliminates this problem. The AccuCyte cell collection process has been proven to recover >90% of targeted CTCs from whole blood, virtually assuring that you will be able to recover the cells you need for your studies.
MOUSE PDX Circulating Tumor Cells
These images show circulating tumor cells obtained from a mouse PDX model, stained with conventional CTC indicators, along with stains for two additional biomarkers.
The RareCyte platform integrates three essential technologies.
- AccuCyte: Comprehensive and highly reliable CTC collection. With the AccuCyte, cell collection is based only on density, which is similar for all nucleated cells. The technology does not rely on cell surface markers, cell lysis, or cell density. These factors can change over time, often eliminating a portion of the CTC population from analysis.
- CyteFinder: Six-channel fluorescence detection. Isolated CTCs can be thoroughly characterized in six fluorescent channels with the companion CyteFinder. Fluorescence analysis is completely independent of cell isolation. This unique design allows the development of highly customized tracking and enumeration schemes tailored to the specific needs of the research.
- CytePicker: Individual CTC isolation. The integrated CytePicker feature facilitates quick and accurate retrieval of individual CTCs of interest. These cells can be prepped for NGS or other
Patient Derived Tumor Xenograft Models
Preinvasive and invasive ductal pancreatic cancer and its early detection in the mouse
Mouse Models in Prostate Cancer Translational Research: From Xenograft to PDX
Patient-derived xenograft models of breast cancer and their predictive power