Question 1

What Orion instrument models are available?

Accepted Answer

RareCyte offers three Orion instrument configurations: the Orion LE, the Orion, and the Orion HT. All three models use the same single-round high-plex whole-slide imaging technology and ArgoFluor reagent system.

Question 2

What is the Orion LE and who is it designed for?

Accepted Answer

The Orion LE is an affordable and streamlined spatial proteomics instrument designed for biomarker panel development, assay optimization, and translational studies. It is well suited for labs establishing a multiplexed immunofluorescence workflow or running targeted panels, and bridges discovery research and pathology with robust performance on clinically relevant FFPE samples.

Question 3

What is the Orion instrument designed for?

Accepted Answer

The Orion is the flagship instrument in the portfolio, delivering 20-channel single-round whole-slide imaging with subcellular resolution. Its 30-slide loader enables walkaway scanning automation, making it ideal for large cohort clinical studies, multi-user environments, and teams running high-volume translational and clinical research programs.

Question 4

What is the Orion HT and who is it designed for?

Accepted Answer

The Orion HT is the highest-throughput instrument in the portfolio, purpose-built for clinical-scale biomarker programs and multi-user environments. It delivers high-plex, high-throughput spatial proteomics with 24/7 hands-off operation enabled by 30-slide automation. Barcode-driven workflows maintain sample custody throughout, and users can continuously add or remove slides and prioritize scan order. It is the only spatial proteomics system that combines 20-channel whole-slide scanning with the accuracy and reproducibility required for clinical trial execution.

Question 5

What applications does the Orion platform support?

Accepted Answer

The Orion platform supports a broad range of spatial biology applications including tumor microenvironment analysis, patient stratification, therapy response assessment, translational biomarker validation, and clinical trial biomarker testing. All models are compatible with FFPE tissue sections and use validated ArgoFluor-conjugated antibodies for reproducible, high-plex results.

Question 6

Can the Orion platform support both discovery research and clinical trials?

Accepted Answer

Yes. Any Orion instrument can support programs from discovery through clinical-scale execution on the same platform. Researchers typically use Orion's cycling capability to run high-plex discovery panels — building and refining biomarker panels across multiple rounds — then transition to a streamlined single-round panel for large cohort clinical studies. This means the same instrument, reagents, and workflow carry a program all the way from spatial biomarker discovery to clinical trial execution without switching platforms.

Specification	Orion HT	Orion	Orion LE
Channels	20	20	14
Slide Capacity	30	2	2
Scan Speed (per cm² per channel)	4 min	4 min	4 min
Whole Slide Scanning	✓	✓	✓
Quantitative Analysis Software	✓	✓	Optional
Imaging Mode	IF, BF	IF, BF	IF
Barcode Workflow	✓	—	—
Ability to Cycle	✓	✓	✓
Storage Capacity	28 TB	28 TB	12 TB
Resolution	4X, 20X (0.325 um)	4X, 20X (0.325 um)	4X, 20X (0.325 um)
Panel Design Tool	✓	✓	✓
Data Management Software	✓	✓	✓

Orion™ Instruments

Spatial Proteomics
Without Compromise

Orion’s innovative 20-channel-per-round-technology unlocks the plex, throughput, and data quality required to scale spatial proteomics into clinical research.

NEW Orion HT

Orion

NEW Orion LE

Designed to support biomarker development at every stage

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