SPATIAL BIOLOGY REALIZED
Orion technology powers highly multiplexed fluorescence imaging and enables comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment in a matter of hours. Accelerate your research with a complete platform of instrumentation, software, and reagents for rapid fluorescence staining and up to 21-channel whole-slide imaging.
ORION SPATIAL BIOLOGY PLATFORM
The Orion platform includes TissuePlex Reagents, the Orion Instrument, and CyteHub software. TissuePlex Reagents are designed for maximum flexibility in developing highly multiplexed tissue panels. Orion is a benchtop, whole-slide fluorescence imaging system that images 21 channels in a single scan within 3 hours and enables rapid spatial insights. CyteHub provides database functionality for easy file management and enables powerful image visualization, annotation, and sharing to facilitate collaboration for highly multiplexed datasets.
Deep multiplexing of a single tonsil section
A tonsil section was stained with TissuePlex reagents and imaged using the Orion Instrument. All 20 biomarkers were stained in a single-staining procedure and imaged in a single scan. Regions of interest (ROI) were selected to show markers of tissue architecture, immune checkpoint, and proliferation in follicles (ROI 1), markers of T-cell subsets (ROI 2), and myeloid and stromal cell markers (ROI 3).
Tonsillar architecture is illustrated by simultaneous display of tonsil B-cell follicles (CD20), inter-follicular T-cell regions (CD3d), blood vessels (SMA and CD31), and crypt mucosal lining (pan-cytokeratin and E-cadherin).
Higher magnification view of squamous epithelium lining the tonsillar crypt. There is E-cadherin staining in basal epithelium, and prominent cytokeratin staining in keratinizing epithelium. In addition to blood vessels, CD31 stains lymphatic stromal cells between the follicle and the mucosa. Rare CD3d+ T-cells are in the epithelium.
B-cell proliferation in follicle germinal centers indicating response to a foreign antigen visualized with PCNA and Ki67. Many orange-yellow staining cells are present indicating co-expression of the red and green markers which are localized to the nucleus.
CD4 and CD8 markers are expressed in non-overlapping subsets of T-cells. Regulatory T-cells (circles) are characterized by FOXP3 expression, which is observed only in the CD4+ cells, and is localized to the nucleus
Non-lymphoid elements of the tonsil follicle. CD11b reveals the follicular dendritic cell network within the follicle. CD11c primarily stains follicular macrophages; CD163 primarily identifies macrophages present in the surrounding T-cell region.
Macrophage subsets. Follicular macrophages express both CD11c and CD68, with a minority at the edge of the follicle expressing CD163. Both CD163 and CD68 staining is present in outside the follicle.
Organization of immune checkpoint markers in the tonsil. PD-L1 expression can be seen in the tonsillar crypt intermixed with E-cadherin. PD-1 is prominently expressed by follicular lymphocytes. Scattered macrophages (CD68) are present in the submucosa and in the follicle.
Immune checkpoint markers in the follicle. Cells expressing PD-1 expression are regularly distributed within the follicle. There is focal PD-L1 expression on CD68+ follicular macrophages (circles).