SPATIAL BIOLOGY REALIZED
Orion technology powers highly multiplexed fluorescence imaging and enables comprehensive phenotypic profiling and spatial analysis of the tissue microenvironment in a matter of hours – even in the presence of tissue autofluorescence. Accelerate your research with a complete platform of instrumentation, software, and reagents for rapid fluorescence staining and 21-channel whole-slide imaging.
ORION SPATIAL BIOLOGY PLATFORM
The Orion platform includes TissuePlex Reagents, the Orion Instrument, and CyteHub software. TissuePlex Reagents are designed for maximum flexibility in developing highly multiplexed tissue panels. Orion is a benchtop, whole-slide fluorescence imaging system that images 21 channels in a single scan within 2 hours and enables rapid spatial insights. CyteHub provides database functionality for easy file management and enables powerful image visualization, annotation, and sharing to facilitate collaboration for highly multiplexed datasets.
Deep multiplexing of a single tonsil section
A tonsil section was stained with TissuePlex reagents and imaged using the Orion Instrument. All markers were stained in a single-staining procedure and imaged in a single scan. In order to best feature relevant biomarkers, two regions of interest (ROI) are shown from the whole-slide, 21-channel scan. ROI #1 includes the tonsillar crypt and B cell follicular region, and ROI #2 includes inter-follicular T cell zone with prominent blood vessels.
In the image at left, markers are pseudocolored as follows: PD-1 (blue), CD4 (purple), CD45RO (yellow), CD20 (cyan), CD3e (magenta), SMA (red), Pan-cytokeratin (green).
|ROI #1 merge pseudocoloring is as follows: CD11b (red), CD45 (green), CD20 (blue), CD68 (gray), CD31 (cyan), E-Cadherin (magenta), and CD11c (yellow). Single channel markers are also shown in grayscale for clarity.|
|ROI #1 merge pseudocoloring is as follows: FOXP3 (red), PD-1 (green), LAG3 (blue), Ki67 (gray), Nuclei (cyan), Pan-CK (magenta), and PCNA (yellow). Single channel markers are also shown in grayscale for clarity.|
|ROI #2 merge pseudocoloring is as follows: SMA (red), PD-L1 (green), CD45RO (blue), CD163 (gray), CD4 (cyan), CD3e (magenta), and CD8a (yellow). Single channel markers are also shown in grayscale for clarity.|