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Neoadjuvant botensilimab plus balstilimab response pattern in locally advanced mismatch repair proficient colorectal cancer

Significant tumor-shrinking activity was observed following neoadjuvant immunotherapy with botensilimab plus balstilimab (BOT/BAL) in microsatellite stable colorectal cancer (MSS CRC). Whole-slide pre-treatment biopsy and post-treatment surgically resected samples were interrogated using one-step, 13-plex imaging on the Orion spatial biology platform. Quantitative analysis was employed to measure immune cell density and proliferation index within and near the tumor for pre-treatment and post-treatment samples. Spatial biology analysis of patient samples can inform mechanisms of action, providing insight for clinical trial designs using neoadjuvant immunotherapy.

Kasi P, Hidalgo M, Jafari M, et al.
Nature Oncogene 2023
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Year	Biopsy type	Classification	Indication	Title	Journal	First Author
2024	Liquid Biopsy	Oncology	Cancer	Circulating tumor cells as liquid biopsy markers in cancer patients	Molecular Aspects of Medicine	Smit D
2024	Liquid Biopsy	Oncology	Cancer	Mesothelin antigen density influences anti-mesothelin chimeric antigen receptor T cell cytotoxicity	Cytotherapy	Chu G
2024	Liquid Biopsy	Oncology	Cancer	Dissemination of Circulating Tumor Cells in Breast and Prostate Cancer: Implications for Early Detection	Endocrinology	Bae S
2024	Liquid Biopsy	NIPT	NIPT	Noninvasive single‐cell‐based prenatal genetic testing: A proof of concept clinical study	Prenatal Diagnosis	Bellair M
2024	Liquid Biopsy	Oncology	Pancreatic cancer	Detection of circulating tumor cells for the early detection of pancreatic cancer	Cancer Research	Yeo D
2024	Liquid Biopsy	Oncology	Breast cancer	Breast Cancer Circulating Tumor Cells: Current Clinical Applications and Future Prospects	Clinical Chemistry	Cani A
2024	Tissue	Oncology	Breast cancer	Qualification of a multiplexed tissue imaging assay and detection of novel patterns of HER2 heterogeneity in breast cancer	npj Breast Cancer	Guerriero J
2023	Liquid Biopsy	Oncology	Colorectal cancer	Circulating tumor cell enumeration, biomarker analyses, and kinetics in patients with colorectal cancer and other GI malignancies	Frontiers in Oncology	Malkawai W
2023	Liquid Biopsy	Oncology	Colorectal cancer	Liquid biopsy kinetics and detection of ERBB2 amplification/HER2-positivity in refractory hepatocellular carcinoma	The Journal of Liquid Biopsy	Gaddem AS
2023	Tissue	Oncology	Colorectal cancer	Neoadjuvant botensilimab plus balstilimab response pattern in locally advanced mismatch repair proficient colorectal cancer	Nature Oncogene	Kasi PM
2023	Tissue	Oncology	Colorectal cancer	High-plex immunofluorescence imaging and traditional histology of the same tissue section for discovering image-based biomarkers	Nature Cancer	Lin JR
2023	Liquid Biopsy	Oncology	Breast cancer	Metastasis from the tumor interior and necrotic core formation are regulated by breast cancer-derived angiopoietin-like 7	PNAS	Yamamoto A
2023	Tissue	Oncology	Colorectal cancer	Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer	Cell	Lin JR
2023	Liquid Biopsy	Oncology	Multiple solid tumor types	Accurate isolation and detection of circulating tumor cells using enrichment-free multiparametric high resolution imaging	Frontiers in Oncology	Yeo D
2022	Liquid Biopsy	Oncology	Multiple solid tumor types	Liquid Biopsy Assessment of Circulating Tumor Cell PD‑L1 and IRF‑1 Expression in Patients with Advanced Solid Tumors Receiving Immune Checkpoint Inhibitor	Targeted Oncology	Kennedy L
2022	Liquid Biopsy	Oncology	Lung adenocarcinoma	Immunofluorescent and molecular characterization of effusion tumor cells reveal cancer site-of-origin and disease-driving mutations	Cancer Cytopatholoty	Zhu Y
2022	Liquid Biopsy	Oncology	Breast cancer	Circulating tumor cell detection: a prospective comparison between CellSearch and Rarecyte platforms in patients with progressive metastatic breast cancer.	Breast Cancer Research and Treatment	Dirix L
2022	Tissue	Oncology	Ovarian cancer	Single-cell tumor-immune microenvironment of BRCA1/2 mutated high-grade serous ovarian cancer	Nature	Lauonen IM
2022	Liquid Biopsy	Oncology	Breast cancer	Safety, Feasibility, and Merits of Longitudinal Molecular Testing of Multiple Metastatic Sites to Inform mTNBC Patient Treatment in the Intensive Trial of Omics in Cancer	JCO Precision Oncology (ASCO)	Burton KA
2022	Liquid Biopsy	Oncology	Multiple solid tumor types	Clinical Validation of a Circulating Tumor Cell Assay Using Density Centrifugation and Automated Immunofluorescence Microscopy	American Journal of Clinical Pathology	Sabath DE
2022	Tissue	Oncology	Melanoma	The Spatial Landscape of Progression and Immunoediting in Primary Melanoma at Single-Cell Resolution	Cancer Discovery	Nirmal AJ
2022	Liquid Biopsy	Oncology	Multiple solid tumor types	Beyond circulating tumor cell enumeration: cell-based liquid biopsy to asses protein biomarkers and cancer genomics	Frontiers	Kaldjian EP
2022	Liquid Biopsy	Immuno-oncology	Prostate cancer	Non-Invasive Profiling of Advanced Prostate Cancer via Multi-Parametric Liquid Biopsy and Radiomic Analysis	International Journal of Molecular Sciences	Morrison G
2022	Liquid Biopsy	Oncology	NSCLC	Single Circulating-Tumor-Cell-Targeted Sequencing to Identify Somatic Variants in Liquid Biopsies in Non-Small-Cell Lung Cancer Patients	Current issues in Molecular biology	Barbirou M
2021	Tissue	Oncology	Multiple solid tumor types	Advancing Spatial Biology through Highly Multiplexed Protein Imaging: The Orion™ Platform	Journal of Precision Medicine	Kaldjian EP
2021	Liquid Biopsy	Oncology	Breast cancer	Heterogeneity of Circulating Tumor Cell Neoplastic Subpopulations Outlined by Single-Cell Transcriptomics	Cancers	Pauken CM
2021	Tissue	Immuno-oncology	Breast cancer	Targeting immunosuppressive macrophages overcomes PARP inhibitor resistance in BRCA1-associated triple-negative breast cancer	Nat Cancer	Mehta AK
2021	Tissue	Oncology	GI cancer	HAND1 and BARX1 Act as Transcriptional and Anatomic Determinants of Malignancy in Gastrointestinal Stromal Tumor	Clinical Cancer Research	Hemming ML
2021	Liquid Biopsy	Oncology	NSCLC	Proficiency Testing to Assess Technical Performance for CTC-Processing and Detection Methods in CANCER-ID	Clinical Chemistry	Neves R
2021	Tissue	Oncology	Synovial sarcoma	Opposing immune and genetic mechanisms shape oncogenic programs in synovial sarcoma	Nat Medicine	Jerby-Arnon L
2017	Liquid Biopsy	Oncology	Multiple solid tumor types	A simple open-source method for highly multiplexed imaging of single cells in tissues and tumours	bioRxiv	Lin JR
2021	Liquid Biopsy	Oncology	Breast cancer	N-Acetyl-L-cysteine Promotes Ex Vivo Growth and Expansion of Single Circulating Tumor Cells by Mitigating Cellular Stress Responses	Molecular Cancer Research	Teng T
2021	Tissue	Immuno-oncology	Melanoma	Evolution of delayed resistance to immunotherapy in a melanoma responder	Nat Medicine	Liu D
2021	Tissue	Immuno-oncology	Brain cancer	Activity of PD-1 blockade with Nivolumab among patients with recurrent atypical/anaplastic meningioma: Phase II trial results	Neuro Oncology	Bi WL
2021	Liquid Biopsy	Oncology	Multiple solid tumor types	Identification and Characterization of Effusion Tumor Cells (ETCs) From Remnant Pleural Effusion Specimens	Cancer Cytopathology	Zhu Y
2020	Liquid Biopsy	Oncology	Prostate cancer	The combination of size-based separation and selection-free technology provides higher circulating tumor cells detection sensitivity than either method alone in patients with metastatic prostate cancer	BJUI International	Dong L
2020	Tissue	Infectious disease	COVID-19	SARS-CoV-2 infection protects against rechallenge in rhesus macaques	Science	Chandrashekar A
2020	Tissue	Oncology	Ovarian cancer	A single-cell landscape of high-grade serous ovarian cancer	Nature Medicine	Izar B
2019	Liquid Biopsy	NIPT	Cervical cancer	A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples	Scientific Reports	Bailey-Hytholt CM
2019	Liquid Biopsy	Oncology	Bladder cancer	Circulating Tumor Cell and Circulating Tumor DNA Assays Reveal Complementary Information for Patients with Metastatic Urothelial Cancer	European Urology Oncology	Chalfin HJ
2019	Tissue	Immuno-oncology	Multiple solid tumor types	Qualifying antibodies for image-based immune profiling and multiplexed tissue imaging	Nature Protocol	Du Z
2019	Liquid Biopsy	Oncology	Breast cancer	PIC&RUN: An integrated assay for the detection and retrieval of single viable circulating tumor cells	Scientific Reports	Kamal M
2019	Liquid Biopsy	NIPT	NIPT	Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis	American Journal of Human Genetics	Vossaert L
2019	Liquid Biopsy	Oncology	Breast cancer	Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis	BMC Cancer	Ramirez AB
2018	Liquid Biopsy	Oncology	Prostate cancer	Prostate Cancer Disseminated Tumor Cells are Rarely Detected in the Bone Marrow of Localized Patients Undergoing Radical Prostatectomy Across Multiple Rare Cell Detection Platforms	The Journal of Urology	Chalfin HJ
2018	Liquid Biopsy	Oncology	Prostate cancer	Optimization of Immunofluorescent Detection of Bone Marrow Disseminated Tumor Cells	Biological Procedures Online	Axelrod HD
2018	Liquid Biopsy	Oncology	Prostate cancer	Analogous detection of circulating tumor cells using the AccuCyte® -CyteFinder® system and ISET system in patients with locally advanced and metastatic prostate cancer	Prostate	van der Toom EE
2018	Liquid Biopsy	Oncology	Bladder cancer	Characterization of urothelial cancer circulating tumor cells with a novel selection-free method	Urology / Genomics in Urologic Health and Disease	Chalfin HJ
2018	Tissue	Immuno-oncology	Multiple solid tumor types	Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes	eLife	Lin JR
2018	Tissue	Immuno-oncology	Melanoma	A Cancer Cell Program Promotes T Cell Exclusion and Resistance to Checkpoint Blockade	Cell	Jerby-Arnon L
2018	Liquid Biopsy	NIPT	NIPT	Reliable detection of subchromosomal deletions and duplications using cell‐based noninvasive prenatal testing	Prenatal Diagnosis	Vossaert L
2018	Liquid Biopsy	Oncology	Multiple solid tumor types	The RareCyte platform for next-generation analysis of circulating tumor cells.	Cytometry Part A	Kaldjian EP
2017	Liquid Biopsy	Oncology	Prostate cancer	Nucleolin staining may aid in the identification of circulating prostate cancer cells	Clinical Genitourinary Cancer	Chalfin HJ
2017	Tissue	Oncology	Melanoma	Adaptive resistance of melanoma cells to RAF inhibition via reversible induction of a slowly dividing de-differentiated state	Molecular Systems Biology	Fallahi-Sichani M
2017	Liquid Biopsy	Oncology	Breast cancer	EMT cells increase breast cancer metastasis via paracrine GLI activation in neighbouring tumour cells	Nature Communications	Neelakantan D
2017	Liquid Biopsy	Oncology	Multiple solid tumor types	RareCyte® CTC Analysis Step 1: AccuCyte® Sample Preparation for the Comprehensive Recovery of Nucleated Cells from Whole Blood	Circulating Tumor Cells: Methods and Protocols, Methods in Molecular Biology	Ramirez AB
2017	Liquid Biopsy	Oncology	Multiple solid tumor types	RareCyte® CTC Analysis Step 2: Detection of Circulating Tumor Cells by CyteFinder® Automated Scanning and Semiautomated Image Analysis	Circulating Tumor Cells: Methods and Protocols, Methods in Molecular Biology	Werbin JL
2017	Liquid Biopsy	Oncology	Multiple solid tumor types	RareCyte® CTC Analysis Step 3: Using the CytePicker® Module for Individual Cell Retrieval and Subsequent Whole Genome Amplification of Circulating Tumor Cells for Genomic Analysis	Circulating Tumor Cells: Methods and Protocols, Methods in Molecular Biology	Stilwell JL
2016	Liquid Biopsy	NIPT	NIPT	Genome-wide copy number analysis on DNA from fetal cells isolated from the blood of pregnant women	Prenatal Diagnosis	Kølvraa S
2016	Liquid Biopsy	Oncology	Breast cancer	A Distributed Network for Intensive Longitudinal Monitoring in Metastatic Triple-Negative Breast Cancer	J. National Comprehensive Cancer Network	Blau CA
2016	Liquid Biopsy	NIPT	NIPT	Evidence for feasibility of fetal trophoblastic cell-based noninvasive prenatal testing	Prenatal Diagnosis	Breman AM
2015	Liquid Biopsy	Oncology	Multiple solid tumor types	High-recovery visual identification and single-cell retrieval of circulating tumor cells for genomic analysis using a dual-technology platform integrated with automated immunofluorescence staining	BMC Cancer	Campton DE
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