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On Demand Webinars

Exploring new frontiers in pancreatic cancer treatment with spatial biology

Speakers:
Yana Zavros, PhD
Professor, University of Georgia School of Medicine

Tad George, PhD
Sr VP Bio R&D, RareCyte

Pancreatic ductal adenocarcinoma (PDAC) remains one of the deadliest cancers, with limited treatment options and a high recurrence rate. Emerging research suggests that the tumor microenvironment (TME) and metastatic site play a critical role in therapy response. In this webinar, Yana Zavros will discuss how she combined spatial biology and patient-derived organoids (PDOs) to uncover a distinct cell population driving therapy resistance and disease recurrence in PDAC.

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Breaking Barriers in Spatial Proteomics with Single-Round Imaging

Speaker
Tad George, PhD
Sr VP Bio R&D, RareCyte

A challenge in multiplex immunofluorescence imaging is the trade-off between multiplexing and throughput. The Orion™ platform overcomes this limitation with 20-channel single-round spatial protein profiling, enabling researchers to perform high-plex spatial biomarker analysis at speeds suitable for large-scale studies.

In this webinar, you'll get an in-depth look at Orion’s capabilities, a walkthrough of its single-round workflow, and customer case studies showcasing key applications. Discover how the Orion platform is setting a new standard in spatial proteomics.

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Accelerating Data and Throughput in Spatial Imaging: A Core Facility Perspective.

Speaker
Zbigniew Mikulski, PhD
Director of Microscopy and Instructor, La Jolla Institute for Immunology

In this webinar, Dr. Mikulski will share best practices from working on over 18 different projects, and how the Orion platform for spatial biology facilitated a fast, low run cost solution that works with FFPE samples. Dr. Mikulski will also discuss practices that other labs might adopt to further improve workflow efficiency and cost-effectiveness.

New discoveries in HER2 detection: A novel method of multimodal detection of HER2 from a single tube of blood

Speaker
Eszter Papp, PhD
R&D Product Manager, CellCarta

In this webinar, Dr. Papp presents a novel method for oncosome detection developed by the CellCarta team using the RareCyte CTC platform. She also presents findings from a proof-of-concept study that combined data from oncosomes, CTCs, and cfDNA analysis to identify metastatic breast cancer patients with elevated HER2 expression.

Use of open-source software for quantitative analysis of multiplex images

Speaker
Dr. Sara McArdle
La Jolla Institute of Immunology

Dr. McArdle discusses her use of open-source software for quantitative spatial analysis of tissue images. She reviews how multiplex immunostaining is a key method for understanding spatial context of the tissue microenvironment and statistical quantification of cells.

Multimodal tissue imaging and machine learning to advance precision medicine

Speaker
Dr. Peter K Sorger
Harvard Medical School

The effective treatment of cancer and many other diseases is increasingly dependent on a precision approach in which the quantification of molecular features at the level of individual patients is used to guide treatment plans. However, cancer diagnosis and staging are currently performed primarily via direct examination of biopsy and resection specimens by histopathologists. These classical methods provide insufficient molecular insight to guide the use of targeted and immunotherapies even when supplemented by knowledge of tumor genotypes.

Spatial analysis and high-plex immunofluorescence to study human pancreata in type 1 diabetes

Speaker
Dr. Estefania Quesada-Masachs
Instructor, La Jolla Institute for Immunology

In this webinar, Dr. Quesada-Masachs will explain the image analysis pipeline that she has developed and applied to study whole tissue pancreatic samples of donors with type 1 diabetes (T1D). Those samples were previously stained and acquired with the high-plex Orion™ platform. New methods for spatial image analysis are providing a better understanding of local processes occurring in many human diseases, revolutionizing those scientific fields.

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Spatial Biology: Applying Cellular Phenotyping in Health and Disease

Speaker
Mickael Meyrand, Field Application Scientist, RareCyte, Inc

Tissue consists of heterogeneous cell types, each with diverse functions and functional states, where spatial organization can impact patient health status. Understanding such spatial context at the subcellular level is often challenging due to low resolution imaging, throughput, and simultaneous target assessment. Orion™ technology can resolve these challenges, providing a fast path to whole slide, multiplexed, and high resolution biomarker imaging data

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Circulating Tumor Cells Circa 2022

Speaker
Daniel E. Sabath, MD, PhD
University of Washington Department of Laboratory Medicine and Pathology

Detecting small numbers of tumor cells is technically challenging, especially for patients with solid tumors, where very small numbers of tumor cells are present in the peripheral blood, even in patients with widely metastatic disease. In this presentation, Dr. Sabath will describe various technologies for detecting circulating tumor cells and data suggesting clinical utility. In particular, his laboratory's clinical validation of a circulating tumor cell assay using density gradient centrifugation to enrich for circulating tumor cells. Dr. Sabath will also discuss possible future applications of this technology.

Unlock Spatial Biology with Orion Technology: Explore the possibilities for research and clinical applications

Speaker
Mickael Meyrand, Field Application Scientist, RareCyte, Inc

Tissue consists of heterogenous cell types, each with diverse functions and functional states, arranged spatially in a way that impacts patient health status. Resolving this complexity at the subcellular level has historically been challenged by image resolution, the number of targets that can be simultaneously assessed, and throughput. Orion™ technology breaks these barriers by providing the fastest path to whole slide, high-plex imaging which will be shown in this webinar.

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Multiplexed Tissue Imaging using the Orion Platform to Reveal the Spatial Biology of Cancer

Speaker
Dr. Sandro Santagata, MD PhD
Associate Professor Pathology, Department of Pathology, at Brigham and Women's Hospital

Recent developments in tissue imaging technologies have made available new tools that promise to advance our understanding of human disease and help guide the development and implementation of new therapies. This interactive webinar will focus on the use of multiplexed antibody-based tissue imaging of multiple antigens at single cell resolution using both cyclic immunofluorescence (CyCIF) and Orion high-plex imaging and the use of this data to identify cell lineages and states (molecular phenotypes) and map their spatial organization and interactions at different scales. 

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Patient Treatment Selection & Disease Monitoring by CTC enumeration

Speakers
Dr. Qingyan Au, Director of Multiplexing, NeoGenomics

Dr. Pashtoon Kasi
Associate Professor of Internal Medicine-Hematology, Oncology and Blood and Marrow Transplantation, University of Iowa

Walla Malkawi, PhD
Graduate student, Pharmaceutics and Drug Delivery, University of Iowa

Tissue consists of heterogenous cell types, each with diverse functions and functional states, arranged spatially in a way that impacts patient health status. Resolving this complexity at the subcellular level has historically been challenged by image resolution, the number of targets that can be simultaneously assessed, and throughput. Orion™ technology breaks these barriers by providing the fastest path to whole slide, high-plex imaging which will be shown in this webinar.

Patient Treatment Selection & Disease Monitoring by CTC enumeration

Speaker
Arturo Ramirez
Director of Oncology R&D

Leighton Howells
Sr VP, Biopharma Partnerships

Recent developments in tissue imaging technologies have made available new tools that promise to advance our understanding of human disease and help guide the development and implementation of new therapies. This interactive webinar will focus on the use of multiplexed antibody-based tissue imaging of multiple antigens at single cell resolution using both cyclic immunofluorescence (CyCIF) and Orion high-plex imaging and the use of this data to identify cell lineages and states (molecular phenotypes) and map their spatial organization and interactions at different scales. 

Resolving the Complexity of Tissue Immune Response with Orion High-Dimensional Imaging

Speaker
Eric Kaldjian, MD
Chief Medical Officer, RareCyte

High-dimensional imaging allows identification of immune cell sub-types for investigation of cell number, density, proximity, and activation state. The breakthrough Orion platform generates same-day whole-slide images with sub-cellular imaging resolution in a single stain, single scan workflow with customizable staining panels. This webinar describes the application of 17-plex immuno-oncology fluorescence panel and H&E images on the same tissue sections for investigation of the complexity of immune response in normal and malignant tissues.

Analytical Validation of CTC Enumeration using the RareCyte Platform and Implementation in a Global Clinical Trial

Speaker
Jeff Fill, MBA, MT(ASCP)
Sr Director, Diagnostic and Experimental Pathology, Eli Lilly

This webinar describes how the RareCyte platform for CTC analysis was validated in the Clinical Diagnostics Lab (CAP accredited CLIA Lab) at Eli Lilly. The results met the requirements of the Clinical Drug Study Team and the RareCyte platform was added to a global clinical trial for further evaluation in collaboration with a CRO. A short presentation on RareCyte CTC assays for enumeration, multi-biomarker analysis, and custom assay development capabilities follows.