Orion Applications

Orion | Orion Instrument | Applications | StrataPlex Reagents

Enable Rapid Spatial Analysis in Research and Clinical Applications

Imaging in up to 21 channels across an entire slide in a single scan enables spatial analysis though examination of biomarker expression, cell classification, and subcellular localization studies to advance pathology, immuno-oncology, infectious disease, and more.

Learn more about the Orion^™ Instrument, the critical technology in the Orion Platform workflow.

Lung tissue

CD68 (blue), CD163 (yellow), CD31 (red), ECad (magenta), CK (white). scale bar = 100 μm

Melanoma tissue

SYTOX (blue), S100 (yellow), Ki67 (red), TIGIT (green). scale bar = 20 μm

Thymus tissue

Vimentin (blue), ECad (magenta), PanCK (green), CAM5.2 (red). scale bar = 200 μm

Tonsil tissue

CD20 (blue), CD4 (white), Ki67 (red), PanCK (yellow). scale bar = 1000 μm

21-channel imaging of lung carcinoma with the Orion Instrument

A single lung section was stained with StrataPlex^™ reagents and imaged using the Orion Instrument. All channels were stained in a single-staining procedure and imaged in a single scan. Three regions of interest (ROIs) are shown below that highlight the channels. Additional single channel images are shown in grayscale for clarity.

Neoplastic Lung

Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).

Merge	CD31	CD45	CD11b

GFAP	CD20	FOXP3	PD-L1

ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1).

Nonneoplastic Lung

Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red).

Merge	Pan-CK	SMA	CD163

E-Cadherin	TTF1	CD4	LAG3

ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3).

Lymphoid Aggregate

Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).

Merge	Ki67	CD11c	CD68

CD3d	CD8a	PD-1	Nuclei

ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1).