Imaging in up to 21 markers across an entire slide in a single scan enables spatial analysis though examination of biomarker expression, cell classification, and subcellular localization studies to advance pathology, immuno-oncology, infectious disease, and more.
Investigate T-cell subsets
Characterize tissue architecture
Examine checkpoint biomarkers
21-marker imaging of lung carcinoma with the Orion Instrument
A single lung section was stained with TissuePlex reagents and imaged using the Orion Instrument. All 21 markers were stained in a single-staining procedure and imaged in a single scan. Three regions of interest (ROIs) are shown below that highlight the 21 markers. Additional single channel images are shown in grayscale for clarity.
Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).
|ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1).|
Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red).
|ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3).|
Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).
|ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1).|