Orion Applications

Enable Rapid Spatial Analysis in Research and Clinical Applications

Imaging in up to 21 channels across an entire slide in a single scan enables spatial analysis though examination of biomarker expression, cell classification, and subcellular localization studies to advance pathology, immuno-oncology, infectious disease, and more.

Learn more about the Orion Instrument, the critical technology in the Orion Platform workflow.

Lung tissue

CD68 (blue), CD163 (yellow), CD31 (red), ECad (magenta), CK (white). scale bar = 100 μm

Melanoma tissue

SYTOX (blue), S100 (yellow), Ki67 (red), TIGIT (green). scale bar = 20 μm

Thymus tissue

Vimentin (blue), ECad (magenta), PanCK (green), CAM5.2 (red). scale bar = 200 μm

Tonsil tissue

CD20 (blue), CD4 (white), Ki67 (red), PanCK (yellow). scale bar = 1000 μm

21-channel imaging of lung carcinoma with the Orion Instrument

A single lung section was stained with StrataPlex reagents and imaged using the Orion Instrument. All channels were stained in a single-staining procedure and imaged in a single scan. Three regions of interest (ROIs) are shown below that highlight the channels. Additional single channel images are shown in grayscale for clarity.

Neoplastic Lung

Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).

Merge CD31 CD45 CD11b
ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1).

Nonneoplastic Lung

Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red). 

Merge Pan-CK SMA CD163
E-Cadherin TTF1 CD4 LAG3
ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3).

Lymphoid Aggregate

Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).

Merge Ki67 CD11c CD68
CD3d CD8a PD-1 Nuclei
ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1).