Imaging in up to 21 markers across an entire slide in a single scan enables spatial analysis though examination of biomarker expression, cell classification, and subcellular localization studies to advance pathology, immuno-oncology, infectious disease, and more.
Visualize micro-anatomy

Investigate T-cell subsets

Characterize tissue architecture

Examine checkpoint biomarkers

21-marker imaging of lung carcinoma with the Orion Instrument
A single lung section was stained with TissuePlex reagents and imaged using the Orion Instrument. All 21 markers were stained in a single-staining procedure and imaged in a single scan. Three regions of interest (ROIs) are shown below that highlight the 21 markers. Additional single channel images are shown in grayscale for clarity.
Neoplastic Lung

Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).
Merge | CD31 | CD45 | CD11b |
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GFAP | CD20 | FOXP3 | PD-L1 |
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ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1). |
Nonneoplastic Lung

Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red).
Merge | Pan-CK | SMA | CD163 |
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E-Cadherin | TTF1 | CD4 | LAG3 |
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ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3). |
Lymphoid Aggregate

Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).
Merge | Ki67 | CD11c | CD68 |
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CD3d | CD8a | PD-1 | Nuclei |
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ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1). |