Orion
Orion Applications

Enable whole slide tissue analysis for Research and Clinical Applications


High-plex imaging across an entire slide, in a single scan, enables spatial analysis through examining biomarker expression, cell classification, and subcellular localization. Such analysis paves a path for advancing studies in pathology, immuno-oncology, infectious disease, and many more areas of disease.

Learn more about the Orion Multiplex Imaging Instrument, the critical technology in the Orion platform workflow.


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Rapid Tissue Analysis for Any Sample

Lung tissue

Normal lung tissue
CD68 (blue), CD163 (yellow), CD31 (red), ECad (magenta), CK (white). scale bar = 100 μm

Skin tissue (melanoma)

Skin Tissue with melanoma
SYTOX (blue), S100 (yellow), Ki67 (red), TIGIT (green). scale bar = 20 μm

Thymus tissue

Thymus tissue
Vimentin (blue), ECad (magenta), PanCK (green), CAM5.2 (red). scale bar = 200 μm

Tonsil tissue

Tonsil tissue
CD20 (blue), CD4 (white), Ki67 (red), PanCK (yellow). scale bar = 1000 μm


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21-plex imaging of lung carcinoma with the Orion Instrument

A single lung section was stained with Orion Reagents and imaged using the Orion instrument. All biomarkers were stained in a single round and imaged in a single scan. Three regions of interest (ROIs) are shown below that highlight the channels. Additional single channel images are shown in grayscale for clarity.


Neoplastic Lung

Neoplastic Lung

Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).

Merge CD31 CD45 CD11b
Neoplastic Lung Neoplastic Lung Neoplastic Lung Neoplastic Lung
GFAP CD20 FOXP3 PD-L1
Neoplastic Lung Neoplastic Lung Neoplastic Lung Neoplastic Lung
ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1).

Non-neoplastic Lung

Non-neoplastic Lung

Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red). 

Merge Pan-CK SMA CD163
Non-neoplastic Lung Non-neoplastic Lung Non-neoplastic Lung Non-neoplastic Lung
E-Cadherin TTF1 CD4 LAG3
Non-neoplastic Lung Non-neoplastic Lung Non-neoplastic Lung Non-neoplastic Lung
ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3).

Lymphoid Aggregate

Lymphoid Aggregate

Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).

Merge Ki67 CD11c CD68
Lymphoid Aggregate Lymphoid Aggregate Lymphoid Aggregate Lymphoid Aggregate
CD3d CD8a PD-1 Nuclei
Lymphoid Aggregate Lymphoid Aggregate Lymphoid Aggregate Lymphoid Aggregate
ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1).




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