Featured Publication

Neoadjuvant botensilimab plus balstilimab response pattern in locally advanced mismatch repair proficient colorectal cancer

Significant tumor-shrinking activity was observed following neoadjuvant immunotherapy with botensilimab plus balstilimab (BOT/BAL) in microsatellite stable colorectal cancer (MSS CRC). Whole-slide pre-treatment biopsy and post-treatment surgically resected samples were interrogated using one-step, 13-plex imaging on the Orion spatial biology platform. Quantitative analysis was employed to measure immune cell density and proliferation index within and near the tumor for pre-treatment and post-treatment samples. Spatial biology analysis of patient samples can inform mechanisms of action, providing insight for clinical trial designs using neoadjuvant immunotherapy.

Kasi P, Hidalgo M, Jafari M, et al.
Nature Oncogene 2023
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Circulating tumor cell enumeration, biomarker analyses, and kinetics in patients with colorectal cancer and other GI malignancies
Malkawi W, Lutfi A, Afghan M, et al.
Frontiers in Oncology 2023
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Liquid biopsy kinetics and detection of ERBB2 amplification/HER2-positivity in refractory hepatocellular carcinoma
Geddam A, Lutfi A, Afghan M, et al.
Journal of Liquid Biopsy 2023
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Accurate isolation and detection of circulating tumor cells using enrichment-free multiparametric high resolution imaging
Yeo D, Kao S, Gupta R, et al.
Frontiers in Oncology 2023
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High-plex immunofluorescence imaging and traditional histology of the same tissue section for discovering image-based biomarkers
Lin J, Chen Y, Campton D, et al.
Nature Cancer 2023
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Shooting for multiplexed pathology with Orion
Anderson A & Gibbs S
Nature Cancer 2023
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HERCULES: A prostate cancer (PC) sequencing panel for parallel analysis of circulating tumor DNA (ctDNA) and circulating tumor cells (CTCs)
Bsteh D, Buckley J, Dejerianne Ostrow D, et al.
Journal of Clinical Oncology 2023
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Metastasis from the tumor interior and necrotic core formation are regulated by breast cancer-derived angiopoietin-like 7
Yamamoto A, Huang Y, Krajina B, et al.
PNAS 2023
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A human breast atlas integrating single-cell proteomics and transcriptomics
Gray G, Li C, Rosenbluth J, et al.
Developmental Cell 2022
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Multiplexed 3D atlas of state transitions and immune interaction in colorectal cancer
Lin J, Wang S, Coy S, et al.
Cell 2022
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The Spatial Landscape of Progression and Immunoediting in Primary Melanoma at Single-Cell Resolution
Nirmal A, Maliga Z, Vallius T, et al.
Cancer Discovery 2022
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Circulating tumor cell detection: A prospective comparison between CellSearch® and RareCyte® platforms in patients with progressive metastatic breast cancer
Dirix L, Buys A, Oeyen S, et al.
Breast Cancer Research and Treatment 2022
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Clinical validation of a circulating tumor cell assay using density centrifugation and automated immunofluorescence microscopy
Sabath D, Perrone M, Clein A, et al.
American Journal of Clinincal Pathology 2022
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Safety, Feasibility, and Merits of Longitudinal Molecular Testing of Multiple Metastatic Sites to Inform mTNBC Patient Treatment in the Intensive Trial of Omics in Cancer
Burton K, Mahen E, Konnick E, et al.
JCO Precision Oncology 2022
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Liquid Biopsy Assessment of Circulating Tumor Cell PD‑L1 and IRF‑1 Expression in Patients with Advanced Solid Tumors Receiving Immune Checkpoint Inhibitor
Kennedy L, Lu J, Kuehn S, et al.
Targeted Oncology 2022
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Non-Invasive Profiling of Advanced Prostate Cancer via Multi-Parametric Liquid Biopsy and Radiomic Analysis
Morrison G, Buckley J, Ostrow D, et al.
Molecular Sciences 2022
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Single-cell tumor-immune microenvironment of BRCA1/2 mutated high-grade serous ovarian cancer
Launonen IM, Lyytikäinen N, Casado J, et al.
Nature Communications 2022
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Beyond Circulating Tumor Cell Enumeration: Cell-Based Liquid Biopsy to Assess Protein Biomarkers and Cancer Genomics Using the RareCyte® Platform
Kaldjian EP, Ramirez AB, Costandy L, et al.
Frontiers in Pharmacology 2022
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Single Circulating-Tumor-Cell-Targeted Sequencing to Identify Somatic Variants in Liquid Biopsies in Non-Small-Cell Lung Cancer Patients
Barbirou M, Miller A, Manjunath Y, et al.
Current Issues in Molecular Biology 2022
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Heterogeneity of Circulating Tumor Cell Neoplastic Subpopulations Outlined by Single-Cell Transcriptomics
Pauken CM, Kenney SR, Brayer KJ, et al.
Cancers 2021
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Identification and Characterization of Effusion Tumor Cells (ETCs) From Remnant Pleural Effusion Specimens
Zhu Y, Allard GM, Ericson NG, George TC, Kunder CA, Lowe AC.
Cancer Cytopathology 2021
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A single-cell landscape of high-grade serous ovarian cancer
Izar B, Tirosh I, Stover EH, et al.
Nature Medicine 2020
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SARS-CoV-2 infection protects against rechallenge in rhesus macaques
Chandrashekar A, Liu J, Martinot AJ, et al.
Science 2020
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Circulating tumor cell investigation in breast cancer patient-derived xenograft models by automated immunofluorescence staining, image acquisition, and single cell retrieval and analysis
Ramirez AB, Bhat R, Sahay D, et al.
BMC Cancer 2019
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PIC&RUN: An integrated assay for the detection and retrieval of single viable circulating tumor cells
Kamal M, Saremi S, Klotz R, et al.
Scientific Repports 2019
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Validation Studies for Single Circulating Trophoblast Genetic Testing as a Form of Noninvasive Prenatal Diagnosis
Vossaert L, Wang Q, Salman R, et al.
American Journal of Human Genetics 2019
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A Rapid Method for Label-Free Enrichment of Rare Trophoblast Cells from Cervical Samples
Bailey-Hytholt C, Sayeed S, Kraus M, et al.
Scientific Repports 2019
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A Cancer Cell Program Promotes T Cell Exclusion and Resistance to Checkpoint Blockade
L Jerby-Arnon, P Shah, M Cuoco, et al.
Cell 2018
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The RareCyte® Platform for Next-Generation Analysis of Circulating Tumor Cells
Kaldjian E, Ramirez A, Sun Y, et al.
Cytometry Part A 2018
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Reliable detection of subchromosomal deletions and duplications using cell-based noninvasive prenatal testing
Vossaert L, Wang Q, Salman R, et al.
Prenatal Diagnostics 2018
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Highly multiplexed immunofluorescence imaging of human tissues and tumors using t-CyCIF and conventional optical microscopes
Lin JR, Izar B, Wang S, et al.
Elife 2018
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Prostate cancer disseminated tumor cells are rarely detected in the bone marrow of patients with localized disease undergoing radical prostatectomy across multiple rare cell detection platforms
Chalfin HJ, Glavaris SA, Malihi PD, et al.
Journal of Urology 2018
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A distributed network for intensive longitudinal monitoring in metastatic triple-negative breast cancer
Blau CA, Ramirez AB, Blau S, et al.
Journal National Comprehensive Cancer Network 2016
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Evidence for feasibility of fetal trophoblastic cell-based noninvasive prenatal testing
Breman AM, Chow JC, U’Ren L, et al.
Prenatal Diagnostics 2016
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High-recovery visual identification and single-cell retrieval of circulating tumor cells for genomic analysis using a dual-technology platform integrated with automated immunofluorescence staining
Campton DE, Ramirez AB, Nordberg JJ, et al.
BMC Cancer 2015
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