Circulating fetal cells (CFCs) can be isolated from maternal blood, and fetal aneuploidies and copy number variants can be evaluated by genetic analysis for research purposes.
The RareCyte® platform enables CFC analysis, including:
- Enumeration of CFCs in maternal blood
- Retrieval of individual CFCs for molecular analysis by comparative genomic hybridization and next generation sequencing
Uniquely RareCyte
Analysis of extremely rare CFCs from blood requires a method that is sensitive, specific, and reproducible, and provides the depth of information you need.
The RareCyte platform provides:
- Sensitivity: Unbiased and highly sensitive target-cell recovery with the AccuCyte® Sample Preparation System
- Specificity: Capture and staining antibodies for isolation and detection of fetal cells, and visual confirmation protects against false positives
- Retrieval: Gentle, single cell isolation for detection of sub-chromosomal duplications and deletions
Representative 40x CFC images with CFC-specific biomarkers shown in green and red.
Cell-based NIPT Workflow
- Prep
- Stain
- Image
- Retrieve
1. Collect nucleated cells from blood and magnetically enrich for CFCs with the CFC Kit
2. Perform immunofluorescence staining with the CFC Kit and pipette onto Chamber slide
3. Identify, enumerate, and phenotype through multiparameter imaging with a CyteFinder® Imaging System
4. Retrieve single CFCs for molecular analysis with the CytePicker® Retrieval Module
