The transformational Orion platform enables same-day highly multiplexed fluorescence analysis of tissue. The platform is comprised of the Orion Instrument, TissuePlex™ Reagents, and CyteHub® software.
- Orion Instrument images 21 channels in a single scan within 2 hours
- Isolates biomarkers from autofluorescence
- Flexible panel development for custom assays
ORION SPATIAL BIOLOGY PLATFORM
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21-channel, whole-slide fluorescence imaging | |
Flexible panel design |
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Stain and image in hours, not days | |
FFPE compatible |
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Single-staining procedure | |
Uses standard microscopy slides |
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High resolution | |
Clinical lab suitability |
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H&E imaging | |
Open file format |
21-marker imaging of lung carcinoma with the Orion Instrument
A single lung section was stained with TissuePlex reagents in a single-staining protocol and imaged using the Orion Instrument in a single scan. Three regions of interest (ROIs) are shown below that highlight the 21 markers. Additional single channel images are shown in grayscale for clarity.
Neoplastic Lung
Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).
| Merge | CD31 | CD45 | CD11b |
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| GFAP | CD20 | FOXP3 | PD-L1 |
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| ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1). | |||
Nonneoplastic Lung
Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red).
| Merge | Pan-CK | SMA | CD163 |
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| E-Cadherin | TTF1 | CD4 | LAG3 |
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| ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3). | |||
Lymphoid Aggregate
Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).
| Merge | Ki67 | CD11c | CD68 |
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| CD3d | CD8a | PD-1 | Nuclei |
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| ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1). | |||
