Orion – Spatial Biology Realized

The transformational Orion platform enables same-day highly multiplexed fluorescence analysis of tissue. The platform is comprised of the Orion Instrument, TissuePlex Reagents, and CyteHub® software.

  • Orion Instrument images 21 channels in a single scan within 2 hours
  • Isolates biomarkers from autofluorescence
  • Flexible panel development for custom assays


21-channel, whole-slide fluorescence imaging Flexible panel design
Stain and image in hours, not days FFPE compatible
Single-staining procedure Uses standard microscopy slides
High resolution Clinical lab suitability
H&E imaging Open file format


21-marker imaging of lung carcinoma with the Orion Instrument

A single lung section was stained with TissuePlex reagents in a single-staining protocol and imaged using the Orion Instrument in a single scan. Three regions of interest (ROIs) are shown below that highlight the 21 markers. Additional single channel images are shown in grayscale for clarity.

Neoplastic Lung

Image above shows cytokeratin-positive, focally TTF1-positive carcinoma in ROI #1. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), SMA (white), and TTF1 (red).

Merge CD31 CD45 CD11b
ROI #1. Single channel images demonstrate blood vessels (CD31), immune cell markers (CD45, CD11b, CD20, FOXP3), and checkpoint biomarker (PD-L1).

Nonneoplastic Lung

Image above shows area of non-neoplastic lung tissue in ROI #2. CD8a (magenta), Nuclei (blue), Pan-cytokeratin (green), and TTF1 (red). 

Merge Pan-CK SMA CD163
E-Cadherin TTF1 CD4 LAG3
ROI #2. Single channel images demonstrate lung epithelial cells (Pan-CK, E-Cadherin, TTF1), blood vessels (SMA), and immune cell markers (CD163, CD4, and LAG3).

Lymphoid Aggregate

Lymphoid aggregate is shown above with germinal center in ROI #3. PD-1 (purple), CD8a (magenta), CD3d (red), CD11b (green), and Ki67 (blue).

Merge Ki67 CD11c CD68
CD3d CD8a PD-1 Nuclei
ROI #3. Single channel images demonstrate proliferation (Ki67), immune cell markers (CD11c, CD68, CD3, and CD8a), and the checkpoint biomarker (PD-1).