Flexible multiplex panel design with Orion™ Reagents
Orion Reagents are tailored to enable spatial biomarker analysis with maximum flexibility. Choose from our range of labeled antibodies to generate Orion spatial biology data for multiplexed tissue imaging, or create new reagents with easy-to-use conjugation kits.
All reagents are compatible with formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections and use a simple staining process based on standard IHC / IF protocols. With ease of staining and ultimate panel flexibility, Orion Reagents are the convenient choice for multiplexed spatial analysis.
Orion Reagent Portfolio
The Orion reagent portfolio of conjugated antibodies and conjugation kits comprises more than 100 products spanning 60 unique biomarkers (human and mouse) and is growing rapidly.
Orion Reagents have enabled the creation of multiple unique panels applied to more than 15 unique tissue types including whole tissue sections and tissue microarrays, encompassing multiple applications including Oncology and Autoimmunity indications.
Type 1 Diabetes
Inflammatory Bowel Disease
Non-Small Cell Lung Cancer
Validated Orion Biomarkers
Select from a broad array of anti-human and anti-mouse primary antibodies directly conjugated to ArgoFluor™ dyes – bright, highly photostable and spectrally separated fluorophores. All Orion reagents are validated for sensitive and specific biomarker quantitation across a range of tissue types and biomarker expression levels. Directly conjugated antibodies enable a simple, one-step staining process and provide best-in-class reproducibility and biomarker quantitation. ArgoFluor dyes are also available as conjugation kits for customers who prefer to label their own reagents.
|Beta III tubulin
|Smooth Muscle Actin
|Beta 2 Microglobulin
Validated Orion Panels – Examples of off-the-shelf panels as used in publications:
Gastrointestinal – Colorectal Cancer (CRC)
Whole-slide tissue section of an invasive colorectal adenocarcinoma stained with a 17-plex immuno-oncology biomarker panel and imaged with the Orion system in a single staining and imaging process.
A Harvard study describes an approach for combining same-slide H&E and high-plex immunofluorescence (IF) data in a whole-slide format to elucidate prognostic biomarkers for CRC patient survival or disease recurrence. Read the publication ➝
Weill Cornell Medicine study study against patient samples in a neoadjuvant trial delivers rapid response information and can inform mechanisms of therapeutic action, providing real-time insight for clinical trial design. Read the study ➝
Pancreas – Type 1 Diabetes (T1D)
Whole-slide tissue section of a pancreas stained with a 16-plex biomarker panel and imaged with the Orion system in a single staining and imaging process. Five of the 16 biomarkers are shown in this image: Hoechst (blue), CD163 (magenta), S100 (white), CD45 (red), CD31 (green).
Watch this webinar where Dr. Quesada-Masachs (La Jolla Institution) describes the image analysis and quantitation pipeline that she applied to study whole tissue pancreatic samples of donors with Type 1 Diabetes with samples stained and imaged with the high-plex Orion platform.
Tongue – Oral Squamous Cell Carcinoma
Whole-slide tissue section of an oral squamous cell carcinoma stained with a 16-plex biomarker panel and imaged with the Orion system in a single staining and imaging process.
Additional tissue types stained with Orion Reagents and imaged on the Orion spatial biology system