Orion
Orion Reagents

Flexible multiplex panel design with Orion Reagents

Orion Reagents are tailored to enable spatial biomarker analysis with maximum flexibility. Choose from our range of labeled antibodies to generate Orion spatial biology data for multiplexed tissue imaging, or create new reagents with easy-to-use conjugation kits.

All reagents are compatible with formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections and use a simple staining process based on standard IHC / IF protocols. With ease of staining and ultimate panel flexibility, Orion Reagents are the convenient choice for multiplexed spatial analysis.


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Orion Reagent Portfolio

The Orion reagent portfolio of conjugated antibodies and conjugation kits comprises more than 100 products spanning 60 unique biomarkers (human and mouse) and is growing rapidly.

Orion Reagents have enabled the creation of multiple unique panels applied to more than 15 unique tissue types including whole tissue sections and tissue microarrays, encompassing multiple applications including Oncology and Autoimmunity indications.



Type 1 Diabetes

Inflammatory Bowel Disease

Non-Small Cell Lung Cancer

Hepatocelluar Carcinoma


View Validated Orion Panels


Validated Orion Biomarkers

Select from a broad array of anti-human and anti-mouse primary antibodies directly conjugated to ArgoFluor dyes – bright, highly photostable and spectrally separated fluorophores. All Orion reagents are validated for sensitive and specific biomarker quantitation across a range of tissue types and biomarker expression levels. Directly conjugated antibodies enable a simple, one-step staining process and provide best-in-class reproducibility and biomarker quantitation. ArgoFluor dyes are also available as conjugation kits for customers who prefer to label their own reagents.

Check back often as our list is always growing or contact us to ask about your biomarkers of interest.
Download the full list ➝



Anti-Human Biomarkers
Albumin
Arginase-1
BCL-6
Beta III tubulin
CD11b
CD11c
CD138
CD14
CD15
CD155
CD163
CD20
CD31
CD3e
CD4
CD40
CD45
CD45RO
CD56
CD68
CD8a
E-Cadherin
FAP
FOLR2
Anti-Human Biomarkers
FOXP3
GATA3
Glucagon
Granzyme B
HLA-ABC
Insulin
Ki-67
LAG3
LRRC15
MYH11
Na, K-ATPase
Neutrophil elastase
Pan-CK
PAX8
PCNA
PD-1
PD-L1
S100a
Smooth Muscle Actin
TBX21
TIGIT
TIM-1
TTF1
Vimentin
Anti-Mouse Biomarkers
Beta 2 Microglobulin
CD3e
CD4
CD8a
CD11c
CD45R (B220)
CD163
E-Cadherin
F4/80
FOXP3
Ki-67
Na, K-ATPase
Pan-CK
PD-L1
Vimentin



Validated Orion Panels – Examples of off-the-shelf panels as used in publications:

Gastrointestinal – Colorectal Cancer (CRC)

Whole-slide tissue section of an invasive colorectal adenocarcinoma stained with a 17-plex immuno-oncology biomarker panel and imaged with the Orion system in a single staining and imaging process.

A Harvard study describes an approach for combining same-slide H&E and high-plex immunofluorescence (IF) data in a whole-slide format to elucidate prognostic biomarkers for CRC patient survival or disease recurrence. Read the publication ➝

Weill Cornell Medicine study study against patient samples in a neoadjuvant trial delivers rapid response information and can inform mechanisms of therapeutic action, providing real-time insight for clinical trial design. Read the study ➝



CRC tissue

Biomarker panel:

  • Nucleus (Hoescht)
  • CD31
  • CD45
  • CD68
  • CD4
  • FOXP3
  • CD8a
  • CD45RO
  • CD20
  • PD-L1
  • CD3e
  • CD163
  • E-Cadherin
  • PD-1
  • Ki-67
  • Pan-CK
  • Smooth Muscle Actin
  • View the full interactive data set ➝




    Pancreas – Type 1 Diabetes (T1D)

    Whole-slide tissue section of a pancreas stained with a 16-plex biomarker panel and imaged with the Orion system in a single staining and imaging process. Five of the 16 biomarkers are shown in this image: Hoechst (blue), CD163 (magenta), S100 (white), CD45 (red), CD31 (green).

    Watch this webinar where Dr. Quesada-Masachs (La Jolla Institution) describes the image analysis and quantitation pipeline that she applied to study whole tissue pancreatic samples of donors with Type 1 Diabetes with samples stained and imaged with the high-plex Orion platform.



    CRC tissue

    Biomarker panel:

  • Nuclear (Hoechst)
  • CD31
  • CD45
  • CD68
  • CD4
  • FOXP3
  • CD8a
  • CD45RO
  • CD20
  • PD-L1
  • CD11c
  • CD163
  • LAG3
  • PD-1
  • Ki67
  • S100



  • Tongue – Oral Squamous Cell Carcinoma

    Whole-slide tissue section of an oral squamous cell carcinoma stained with a 16-plex biomarker panel and imaged with the Orion system in a single staining and imaging process.


    Oral SCC

    Biomarker panel:

  • Nuclear (Hoechst)
  • CD31
  • CD45
  • Vimentin
  • CD4
  • FOXP3
  • CD8a
  • MYH11
  • CD20
  • FAP
  • CD3e
  • E-Cadherin
  • PD-1
  • Ki-67
  • Pan-CK
  • Smooth Muscle Actin
  • View the full interactive data set ➝




    Additional tissue types stained with Orion Reagents and imaged on the Orion spatial biology system

    skin tissue
    Skin (Melanoma)
    thymus tissue
    Thymus
    Tonsil tissue
    Tonsil
    lymph node
    Lymph Node




    Explore more imaging in our interactive data set viewer

    View an interactive data set

    View an interactive dataset