Flexible multiplex panel design

Validated Orion Biomarkers

Select from a broad array of anti-human and anti-mouse primary antibodies directly conjugated to ArgoFluor^™ dyes – bright, highly photostable and spectrally separated fluorophores. All Orion biomarkers are validated for sensitive and specific biomarker quantitation across a range of tissue types and expression levels. Directly conjugated antibodies enable a simple, one-step staining process and provide best-in-class reproducibility and biomarker quantitation. ArgoFluor dyes are also available as conjugation kits for customers who prefer to label their own biomarkers.

Check back often as our list is always growing or contact us to ask about your biomarkers of interest.
Download the full list ➝

Customizable Orion panels built from validated Orion biomarkers span multiple tissues and applications

Gastrointestinal – Colorectal Cancer (CRC)

This is a sample of Orion imaging utilizing an Orion biomarker panel. Whole-slide tissue section of an invasive colorectal adenocarcinoma was stained with a 17-plex immuno-oncology biomarker panel and imaged with the Orion system in a single staining and imaging process.

A Harvard study describes an approach for combining same-slide H&E and high-plex immunofluorescence (IF) data in a whole-slide format to elucidate prognostic biomarkers for CRC patient survival or disease recurrence. Read the publication ➝

This Weill Cornell Medicine study observes patient samples in a neoadjuvant trial delivering rapid response information and shows potential to inform mechanisms of therapeutic action, providing real-time insight for clinical trial design. Read the study ➝

Biomarker panel:

Pancreas – Type 1 Diabetes (T1D)

Whole-slide tissue section of a pancreas stained with a 16-plex biomarker panel and imaged with the Orion system in a single staining and imaging process. Five of the 16 biomarkers are shown in this image: Hoechst (blue), CD163 (magenta), S100 (white), CD45 (red), CD31 (green).

Watch this webinar where Dr. Quesada-Masachs (La Jolla Institution) describes the image analysis and quantitation pipeline that she applied to study whole tissue pancreatic samples of donors with Type 1 Diabetes with samples stained and imaged with the high-plex Orion platform.

Biomarker panel:

Breast ductal adenocarcinoma

Whole-slide tissue with same section H&E imaging of a high-grade breast ductal adenocarcinoma. The tumor is infiltrated by T cells and macrophages.

Biomarker panel:

NSCLC-adjacent Lung

Whole-slide scan of a single section of lung from a region adjacent to a non-small cell lung carcinoma. It has intact normal lung alveolar architecture, with scattered immune cell infiltrates.

Biomarker panel:

Orion Reagent Portfolio

Orion Reagents are tailored to enable spatial biomarker analysis with maximum flexibility. Choose from our range of labeled antibodies to generate Orion spatial biology data for multiplexed tissue imaging, or create new reagents with easy-to-use conjugation kits. All reagents are compatible with formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections and use a simple staining process based on standard IHC / IF protocols. With ease of staining and ultimate panel flexibility, Orion Reagents are the convenient choice for multiplexed spatial analysis.

Explore more imaging in our interactive data set viewer