Orion
Orion Panels

Flexible multiplex panel design


The Orion biomarker portfolio comprises of more than 100 products and enable easy, custom panel design applied to more than 15 unique tissue types, including whole tissue sections and tissue microarrays, across multiple applications including Oncology and Autoimmunity indications.


Type 1 Diabetes

Inflammatory Bowel Disease

Non-Small Cell Lung Cancer

Hepatocelluar Carcinoma


skin tissue
Skin (Melanoma)
thymus tissue
Thymus
Tonsil tissue
Tonsil
lymph node
Lymph Node


Validated Orion Biomarkers

Select from a broad array of anti-human and anti-mouse primary antibodies directly conjugated to ArgoFluor dyes – bright, highly photostable and spectrally separated fluorophores. All Orion biomarkers are validated for sensitive and specific biomarker quantitation across a range of tissue types and expression levels. Directly conjugated antibodies enable a simple, one-step staining process and provide best-in-class reproducibility and biomarker quantitation. ArgoFluor dyes are also available as conjugation kits for customers who prefer to label their own biomarkers.

Check back often as our list is always growing or contact us to ask about your biomarkers of interest.
Download the full list ➝



Human Biomarkers
CD3e
CD4
CD8a
CD11b
CD11c
CD14
CD15
CD16
CD20
CD31
CD45
CD45RO
CD56
CD68
CD69
CD123
CD141
CD163
E-cadherin
ER
FAP
FOLR2
FOXP3
GATA3
Human Biomarkers
Glucagon
Granzyme B
HER2
HLA-ABC
HLA-DR
IL-4I1
Insulin
Ki-67
LAG3
Neutrophil elastase
Pan-CK
PAX8
PD-1
PD-L1
PR
S100a
SOX10
SMA
SPP1
TBX21
TREM2
TIGIT
Vimentin
Mouse Biomarkers
Beta 2 Microglobulin
CD3e
CD4
CD8a
CD11c
CD45R (B220)
CD163
E-Cadherin
F4/80
FOXP3
Ki-67
Na, K-ATPase
Pan-CK
PD-1
PD-L1
Vimentin



Customizable Orion panels built from validated Orion biomarkers span multiple tissues and applications

Gastrointestinal – Colorectal Cancer (CRC)

Whole-slide tissue section of an invasive colorectal adenocarcinoma stained with a 17-plex immuno-oncology biomarker panel and imaged with the Orion system in a single staining and imaging process.

A Harvard study describes an approach for combining same-slide H&E and high-plex immunofluorescence (IF) data in a whole-slide format to elucidate prognostic biomarkers for CRC patient survival or disease recurrence. Read the publication ➝

Weill Cornell Medicine study study against patient samples in a neoadjuvant trial delivers rapid response information and can inform mechanisms of therapeutic action, providing real-time insight for clinical trial design. Read the study ➝



 

CRC tissue

 

Biomarker panel:

  • Nucleus (Hoescht)
  • CD31
  • CD45
  • CD68
  • CD4
  • FOXP3
  • CD8a
  • CD45RO
  • CD20
  • PD-L1
  • CD3e
  • CD163
  • E-Cadherin
  • PD-1
  • Ki-67
  • Pan-CK
  • Smooth Muscle Actin

View the full interactive data set ➝




Pancreas – Type 1 Diabetes (T1D)

Whole-slide tissue section of a pancreas stained with a 16-plex biomarker panel and imaged with the Orion system in a single staining and imaging process. Five of the 16 biomarkers are shown in this image: Hoechst (blue), CD163 (magenta), S100 (white), CD45 (red), CD31 (green).

Watch this webinar where Dr. Quesada-Masachs (La Jolla Institution) describes the image analysis and quantitation pipeline that she applied to study whole tissue pancreatic samples of donors with Type 1 Diabetes with samples stained and imaged with the high-plex Orion platform.



 

CRC tissue

 

Biomarker panel:

  • Nuclear (Hoechst)
  • CD31
  • CD45
  • CD68
  • CD4
  • FOXP3
  • CD8a
  • CD45RO
  • CD20
  • PD-L1
  • CD11c
  • CD163
  • LAG3
  • PD-1
  • Ki67
  • S100



Breast ductal adenocarcinoma

Whole-slide tissue with same section H&E imaging of a high-grade breast ductal adenocarcinoma. The tumor is infiltrated by T cells and macrophages.



 

CRC tissue

 

Biomarker panel:

  • Nucleus (Hoescht)
  • Autofluorescence
  • Pan-CK
  • CD31
  • CD163
  • CD4
  • Ki-67
  • E-Cadherin
  • CD45
  • SMA
  • CD8a
  • CD68
  • CD20
  • FOXP3
  • PD-L1
  • PD-1

View the full interactive data set ➝




NSCLC-adjacent Lung

Whole-slide scan of a single section of lung from a region adjacent to a non-small cell lung carcinoma. It has intact normal lung alveolar architecture, with scattered immune cell infiltrates.



 

CRC tissue

 

Biomarker panel:

  • Nucleus (SYTOX Blue)
  • Autofluorescence
  • CD31
  • CK-CAM5.2
  • CD68
  • CD163
  • Pan-CK
  • CD3d
  • CD20
  • CD4
  • CD8a
  • FOXP3
  • Ki67
  • PCNA
  • CD45RO
  • Vimentin
  • E-Cadherin

View the full interactive data set ➝










Orion Reagent Portfolio

Orion Reagents are tailored to enable spatial biomarker analysis with maximum flexibility. Choose from our range of labeled antibodies to generate Orion spatial biology data for multiplexed tissue imaging, or create new reagents with easy-to-use conjugation kits. All reagents are compatible with formalin-fixed paraffin-embedded (FFPE) or frozen tissue sections and use a simple staining process based on standard IHC / IF protocols. With ease of staining and ultimate panel flexibility, Orion Reagents are the convenient choice for multiplexed spatial analysis.


Explore more imaging in our interactive data set viewer

View an interactive data set

View an interactive dataset