Single-Cell Retrieval and Molecular Analysis

Single-cell analysis allows exploration of cell types and states present in health and disease. Key drivers of biologic processes often are found in rare-cell subsets, and whole population analysis obscures rare-cell data.

Analysis of rare, single cells demands accurate image-based identification of cell type, reliable single-cell isolation, preservation of nucleic acid integrity, and workflows compatible with RNA and DNA analysis.

RareCyte technology enables molecular analysis of rare, single cells, including:

  • Mutational analysis and detection of sub-chromosomal duplications and deletions
  • RNA sequencing-based gene expression analysis
  • T-cell antigen receptor discovery

Uniquely RareCyte

  • Sensitivity: Platform tailored for ultra-rare cell collection, identification, and isolation
  • Specificity: Protein expression heterogeneity among rare target cells revealed with up to six channels, and image-based cell identification and selection ensures integrity of single cell data
  • Retrieval: Gentle single cell isolation from multiple sample preparation types that enable both DNA and RNA analysis
Monitor changes in CTC count (top) and mutational burden (bottom) over time in triple-negative breast cancer patients
ITOMIC-001 study data courtesy of Tony Blau, University of Washington, Anup Madan and Kellie Howard, Covance Genomics
Obtain TCR sequence from individual T cells retrieved with the CytePicker® Module.
Differential expression analysis of activated versus control T cells identifies clusters involved in T-cell signaling and inflammation.

Contact us to learn more about single-cell retrieval and molecular analysis →

CytePicker® Module picks a single cell from AccuCyte® slide

Single cell is deposited into PCR imaging tube

Deposition is visually confirmed by imaging on CyteFinder® Instrument